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1 2 3 Crompton, D. W. T. 1988 ; Parasitol. Today 4, 162169 Whitfield, P. J. 1993 ; in Modern Parasitology Cox, F. E. G., ed. ; , pp. 2452, Blackwell Scientific Publications, Oxford Clark, A. G. 1989 ; Comp. Biochem. Physiol. 92, 419446 Clark, A. G. 1990 ; in Glutathione S-Transferases and Drug Resistance Hayes, J. D., Pickett, C. B. and Mantle, T. J., eds. ; , pp. 369378, Taylor and Francis, London Shah, D. M., Hironaka, C. M., Wiegand, R. C., Harding, E. I., Krivi, G. G. and Tiemeier, D. C. 1986 ; Plant Mol. Biol. 6, 203211 Brophy, P. M. and Barrett, J. 1990 ; Parasitology 100, 345349 Moscow, J. A. and Dixon, K. H. 1993 ; Cytotechnology 12, 155170 Liebau, E., Scho$ nberger, O. L., Walter, R. D. and Henkle-Duhrsen, K. J. 1994 ; $ Mol. Biochem. Parasitol. 63, 167170 Mannervik, B. and Guthenberg, C. 1981 ; Methods Enzymol. 77, 231235 Jaffe, J. J. and Lambert, R. A. 1986 ; Mol. Biochem. Parasitol. 20, 199206 H Alin, P., Danielson, U. H. and Mannervik, B. 1985 ; FEBS Lett. 197, 267270 Gunzler, W. A. and Flohe, L. 1987 ; in Handbook of Methods for Oxygen Radical $ Research, Greenwald, R. A., ed. ; , pp. 285289, CRC Press, Inc., Boca Raton, FL, U.S.A. Brophy, P. M. and Barrett, J. 1987 ; Biochem. Soc. Trans. 15, 1105 Bradford, M. M. 1976 ; Anal. Biochem. 72, 248254 Brophy, P. M. and Barrett, J. 1990 ; Parasitology 100, 345349 Cordell, J. L., Falini, B., Erber, W. N., Ghosh, A. K., Abdulaziz, Z., Macdonald, S., Pulford, K. H. F., Stein, H. and Mason, D. Y. 1984 ; J. Histochem. Cytochem. 32, 219229 Jowett, T., Rizki, T. M. and Rizki, R. M. 1986 ; Dev. Biol. 116, 2330 and misoprostol.
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J.N.W. and J.S. was supported in part by the National Institutes of Health Intramural Targeted AIDS Antiviral Program. Partial support was also received from NIH grant 26356 from the AIDS program of the National Institute of Allergy and Infectious Diseases. C.F. is the recipient of a faculty career development award in clinical pharmacology from the Pharmaceutical Manufacturers Association Foundation. We acknowledge the important technical contributions of Diana Barnes, Kathleen Bell, Barbara Flood, Joe Slusher, Vera Svobodova, Barbara Taverna, Carolyn Wachter, and the personnel of the HIV Unit and Clinical Investigation Directorate of the Wilford Hall Medical Center, because cytotec miscarriage.
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Fc-dependent granulocyte activation is required for blister induction by IgA1 autoantibodies to the cleaved ectodomain of BP180 Type XVII collagen C Sitaru, 1 A Kromminga, 2 GJ Oostingh, 1 E Brocker 1 and D Zillikens 1 Dept. of Dermatology, Univ. of Wuerzburg, Wuerzburg, Germany and 2 IPM, Hamburg, Germany IgA plays an important role in immune exclusion at mucosal surfaces and, by binding to its receptor on myeloid cells, triggers a plethora of effector functions. While IgA is important for the immune defence, IgA autoantibodies are associated with various autoimmune conditions. However, a direct pathogenic role of IgA autoantibodies has not yet been demonstrated. Linear IgA disease LAD ; , an autoimmune subepidermal blistering disease, is associated with IgA autoantibodies to BP180 type XVII collagen. These autoantibodies preferentially bind to the cleaved 120 kDa ectodomain of this protein. The aim of the present study was to investigate the blister-inducing potential of IgA autoantibodies in LAD. Interestingly, serum from patients with LAD n 10 ; , but not from normal controls, induced subepidermal splits in cryosections of human skin when co-incubated with leukocytes from healthy volunteers. IgA1 fractions of LAD patients sera, purified by Jacalin affinity chromatography, retained the blister inducing capacity, while serum depleted of IgA1 was not pathogenic. Recruitment of granulocytes at the dermal-epidermal junction, but not of peripheral blood mononuclear cells, was a prerequisite for split induction. These granulocytes were activated as revealed by their capacity to reduce nitro blue tetrazolium to formazan. F ab ; 2 fragments of patients IgA, lacking the effector Fc portion, lost their split-inducing capacity. Importantly, preadsorption of IgA autoantibodies against the shed ectodomain, but not against a recombinant form of full-length type XVII collagen, abolished their blister-inducing potential. In summary, for the first time, we demonstrate the pathogenicity of autoantibodies of the IgA isotype. In addition, our findings suggest that epitopes, generated by cleavage of the type XVII collagen ectodomain, are important for binding of blister-inducing IgA autoantibodies to this target antigen.
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Their own physicians. The primary outcome variable was patient's overall status compared to baseline on a 7 point Likert scale range 1 much worse, 7 much better ; . Secondary outcome measures included measurements for pain, fatigue, and patient and physician global assessment on a visual analog scale. Additional functional measures were the disease-specific Fibromyalgia Impact Questionnaire FIQ ; , and the generic Health Assessment Questionnaire HAQ ; . RESULTS: Of 70 85% ; patients who were followed up at 3 years, 33 47% ; reported overall moderate to marked improvement, and the remaining 53% reported either slight improvement, no change, or deterioration. The improved group n 33 ; compared to those that remained the same or worsened n 37 ; showed significant differFibromyalgia Abstracts: December 2003.
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